Metagenomics sample preparation and sequencing.
نویسندگان
چکیده
منابع مشابه
An automated sample preparation system for large-scale DNA sequencing.
Recent advances in DNA sequencing technologies, both in the form of high lane-density gels and automated capillary systems, will lead to an increased requirement for sample preparation systems that operate at low cost and high throughput. As part of the development of a fully automated sequencing system, we have developed an automated subsystem capable of producing 10,000 sequence-ready ssDNA t...
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A major limitation for better understanding the role of the human gut virome in health and disease is the lack of validated methods that allow high throughput virome analysis. To overcome this, we evaluated the quantitative effect of homogenisation, centrifugation, filtration, chloroform treatment and random amplification on a mock-virome (containing nine highly diverse viruses) and a bacterial...
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BACKGROUND Different high-throughput nucleic acid sequencing platforms are currently available but a trade-off currently exists between the cost and number of reads that can be generated versus the read length that can be achieved. METHODOLOGY/PRINCIPAL FINDINGS We describe an experimental and computational pipeline yielding millions of reads that can exceed 200 bp with quality scores approac...
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Errors made during the sample preparative process contribute significantly to the total analytical error of immunoassays. Reduction in the magnitude of these errors have been made by improvements in the manualfluid handling systems and mechanization of the individual steps in the process. The mechanization has been stimulated by developments in both computing hardware and software. A laboratory...
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1Nuclear Fuel Cycle Research School, Nuclear Science & Technology Research Institute, Atomic Energy Organization of Iran, P.O. Box 14395-836, Tehran, Iran 2Department of Chemistry, College of Basic Science, Islamic Azad University, Yadegar-e-Imam Khomeini (RAH) Branch, P.O. Box 18155-144, Tehran, Iran 3Department of Chemistry, K.N. Toosi University of Technology, P.O. Box 15875-4416, Tehran, Ir...
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ژورنال
عنوان ژورنال: EMBnet.journal
سال: 2013
ISSN: 2226-6089
DOI: 10.14806/ej.19.a.662